Fig. 3. Extracellular cytokine production in γ-Res-treated BMDCs and DCs generated by γ-Res in the presence of LPS. A) BMDCs were treated with intact resveratrol (30 μg/mL) or γ-Res (30 μg/mL) with LPS for 24 h, and then, the TNF-α, IL-12p70 and IL-10 levels in the culture medium were analyzed by ELISA. B) BMDCs and γ-Res-DCs were treated with LPS (100 ng/mL) for 24 h, and then, the cytokine levels in the culture medium were analyzed by ELISA. C) LPS-treated BMDCs were treated with γ-Res at various doses (1, 15, and 30 μg/mL), and then, the cytokine levels in the culture medium were analyzed by ELISA. The results are representative of three independent experiments. All data were expressed as the means ± SD (n = 4 samples per group). Con: untreated BMDCs, LPS+Intact-Res: BMDC treated with LPS and intact resveratrol, LPS+γ-Res: BMDC treated with LPS and γ-Res. ^*p<0.05, ^**p<0.01, or ^***p<0.001. n.s., no significant effect.